EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.27 | - |
Homo sapiens |
1.6.5.5 | expressed in Escherichia coli as a His-tagged fusion protein | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.3.1.27 | to 1.85 A resolution. The overall structure displays the typical medium-chain dehydrogenase fold with two domains: the catalytic domain, residues 1-128 and 271-329, and the coenzyme-binding domain, residues 129-270, which are separated by a deep cleft that binds the cofactor NADP+ and the substrate | Homo sapiens |
1.6.5.5 | crystallographic structure of human zeta-crystallin is reported. Enzyme shows a tetrameric structure | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.1.27 | Y53F | substrate-pocket residue, large increase in Km values for all substrates and the cofactor, but mainly towards 3-buten-2-one and propenal with a 30fold increase | Homo sapiens |
1.3.1.27 | Y59F | substrate-pocket residue, almost the same kinetic parameter values as the wild-type enzyme for 2-alkenals | Homo sapiens |
1.6.5.5 | T53F | Tyr53Phe mutant displays a large increase in Km values for all substrates and the cofactor, but mainly towards 3-buten-2-one and propenal with a 30fold increase. For 3-penten-2-one, 3-nonen-2-one, 2-hexenal and 4-hydroxy-2-hexenal mutant also shows a decrease in kcat | Homo sapiens |
1.6.5.5 | T59F | Tyr59Phe mutant exhibits almost the same kinetic parameter values as the wild-type enzyme for 2-alkenals,while the Km is increased for 4-hydroxy-2-hexenal | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.27 | 0.005 | - |
NADPH | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.3.1.27 | 0.015 | - |
NADPH | mutant Y59F, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.3.1.27 | 0.035 | - |
3-buten-2-one | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.3.1.27 | 0.06 | - |
NADPH | mutant Y53F, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.3.1.27 | 0.13 | - |
4-hydroxy-2-hexenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.3.1.27 | 0.48 | - |
2-nonenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.3.1.27 | 0.55 | - |
4-hydroxy-2-nonenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.3.1.27 | 1.26 | - |
4-hydroxy-2-hexenal | mutant Y59F, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.3.1.27 | 3.67 | - |
4-hydroxy-2-hexenal | mutant Y53F, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.6.5.5 | 0.005 | - |
NADPH | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 0.015 | - |
NADPH | pH 7, 25°C, mutant T59F | Homo sapiens | |
1.6.5.5 | 0.035 | - |
3-buten-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 0.05 | - |
3-nonen-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 0.06 | - |
NADPH | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 0.06 | - |
3-penten-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 0.06 | - |
3-buten-2-one | pH 7, 25°C, mutant T59F | Homo sapiens | |
1.6.5.5 | 0.08 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 0.13 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 0.13 | - |
3-buten-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 0.13 | - |
2-hexenal | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 0.482 | - |
2-nonenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 0.535 | - |
3-penten-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 0.55 | - |
4-hydroxy-2-nonenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 0.86 | - |
propenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
1.6.5.5 | 0.94 | - |
3-nonen-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 1.26 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
1.6.5.5 | 1.45 | - |
propenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 2.3 | - |
propenal | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 5.45 | - |
2-hexenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 6.12 | - |
2-hexenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
1.6.5.5 | 8.6 | - |
2-pentenal | pH 7, 25°C, wild-type | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.27 | Homo sapiens | Q08257 | - |
- |
1.6.5.5 | Homo sapiens | Q08257 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.6.5.5 | using Ni-NTA chromatography | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.27 | 2-nonenal + NADPH + H+ | - |
Homo sapiens | nonanal + NADP+ | - |
ir | |
1.3.1.27 | 3-buten-2-one + NADPH + H+ | - |
Homo sapiens | butan-2-one + NADP+ | - |
? | |
1.3.1.27 | 4-hydroxy-2-hexenal + NADPH + H+ | - |
Homo sapiens | 4-hydroxyhexadecanal + NADP+ | - |
? | |
1.3.1.27 | 4-hydroxy-2-nonenal + NADPH + H+ | - |
Homo sapiens | 4-hydroxynonanal + NADP+ | - |
? | |
1.3.1.27 | additional information | enzyme additionally catalyzes the reduction of quinones, reaction of EC 1.6.5.5. The enzyme shows a low level of activity with cinnamaldehyde and no activity with 2-cyclohexen-1-one and 15-ketoprostaglandin E2. No activity is detected with nonanal and 2-nonene | Homo sapiens | ? | - |
? | |
1.6.5.5 | 2-hexenal + NADPH | - |
Homo sapiens | hexanal + NADP+ | - |
? | |
1.6.5.5 | 2-nonenal + NADPH | - |
Homo sapiens | nonanal + NADP+ | - |
? | |
1.6.5.5 | 2-pentenal + NADPH | - |
Homo sapiens | pentanal + NADP+ | - |
? | |
1.6.5.5 | 3-buten-2-one + NADPH | - |
Homo sapiens | 2-butanone + NADP+ | - |
? | |
1.6.5.5 | 3-nonen-2-one + NADPH | - |
Homo sapiens | 2-nonanone + NADP+ | - |
? | |
1.6.5.5 | 3-penten-2-one + NADPH | - |
Homo sapiens | 2-pentanone + NADP+ | - |
? | |
1.6.5.5 | 4-hydroxy-2-hexenal + NADPH | - |
Homo sapiens | 4-hydroxy-hexanal + NADP+ | - |
? | |
1.6.5.5 | 4-hydroxy-2-nonenal + NADPH | - |
Homo sapiens | 4-hydroxy-nonanal + NADP+ | - |
? | |
1.6.5.5 | propenal + NADPH | - |
Homo sapiens | propanal + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.6.5.5 | dimer | gel filtration, 3 M NaCl | Homo sapiens |
1.6.5.5 | homotetramer | gel filtration, 0.3 M NaCl | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.27 | zeta-Crystallin | - |
Homo sapiens |
1.6.5.5 | zeta-Crystallin | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.27 | 0.113 | - |
2-nonenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.3.1.27 | 0.167 | - |
3-buten-2-one | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.3.1.27 | 1.15 | - |
4-hydroxy-2-nonenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.3.1.27 | 1.33 | - |
4-hydroxy-2-hexenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.6.5.5 | 0.11 | - |
2-nonenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 0.16 | - |
3-buten-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 0.2 | - |
3-penten-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 0.2 | - |
2-pentenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 0.21 | - |
3-nonen-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 0.28 | - |
2-hexenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 0.28 | - |
3-buten-2-one | pH 7, 25°C, mutant T59F | Homo sapiens | |
1.6.5.5 | 0.36 | - |
2-hexenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
1.6.5.5 | 0.97 | - |
3-buten-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 1.15 | - |
4-hydroxy-2-nonenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 1.25 | - |
propenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
1.6.5.5 | 1.33 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 1.66 | - |
propenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 2.16 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
1.6.5.5 | 2.66 | - |
3-penten-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 3.11 | - |
3-nonen-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 3.67 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 39.74 | - |
propenal | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 73.27 | - |
2-hexenal | pH 7, 25°C, mutant T53F | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.27 | NADPH | - |
Homo sapiens | |
1.6.5.5 | NADPH | - |
Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.27 | 0.02 | - |
4-hydroxy-2-hexenal | mutant Y53F, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.3.1.27 | 0.23 | - |
2-nonenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.3.1.27 | 1.67 | - |
4-hydroxy-2-hexenal | mutant Y59F, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.3.1.27 | 2.1 | - |
4-hydroxy-2-nonenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.3.1.27 | 4.8 | - |
3-buten-2-one | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.3.1.27 | 10.2 | - |
4-hydroxy-2-hexenal | wild-type, pH 7.5, temperature not specified in the publication | Homo sapiens | |
1.6.5.5 | 0.0016 | - |
2-hexenal | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 0.016 | - |
3-nonen-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 0.023 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 0.023 | - |
2-pentenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 0.025 | - |
3-penten-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 0.051 | - |
2-hexenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 0.058 | - |
propenal | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 0.06 | - |
2-hexenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
1.6.5.5 | 0.13 | - |
3-buten-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.6.5.5 | 0.23 | - |
2-nonenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 0.23 | - |
3-nonen-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 0.36 | - |
3-penten-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 1.16 | - |
propenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 1.5 | - |
propenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
1.6.5.5 | 1.66 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
1.6.5.5 | 2.1 | - |
4-hydroxy-2-nonenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 4.6 | - |
3-buten-2-one | pH 7, 25°C, mutant T59F | Homo sapiens | |
1.6.5.5 | 4.8 | - |
3-buten-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
1.6.5.5 | 10.21 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, wild-type | Homo sapiens |